Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

14.1K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
14.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scalable multiplexed machine learning gas sensor chips for food classification.

Science advances·2026
Same author

Wireless, battery-free wearable optoelectronic-colorimetric microfluidic sensor for multiplex sweat analysis.

Device·2026
Same author

New generic classification for Korean Peucedanum L. (Apiaceae) species based on deep phylogenomic study.

BMC plant biology·2026
Same author

Genetic dissection and transcriptomic analysis of a novel high-tillering phenotype in rice derived from weedy rice (Hapcheonaengmi3) and Tongil-type Rice (Milyang23).

The plant genome·2026
Same author

Versatile water-floated nanostructures for three-dimensional nanotransfer printing.

Nature communications·2026
Same author

Biologically inspired microlens array camera for high-resolution wide field-of-view imaging.

Nature communications·2026

Related Experiment Video

Updated: May 4, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K

Quantum dot-based immunoassay enhanced by high-density vertical ZnO nanowire array.

Jung Kim1, Seyong Kwon2, Je-Kyun Park3

  • 1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.

Biosensors & Bioelectronics
|January 4, 2014
PubMed
Summary

This study presents a novel immunoassay platform using zinc oxide (ZnO) nanowires and quantum dots (QDs) for enhanced biomolecule detection. The platform achieves high sensitivity and a broad detection range for biomarkers like carcinoembryonic antigen (CEA).

Keywords:
BiosensingCarcinoembryonic antigenFluorescence resonance energy transferImmunoassayQuantum dotZnO nanowire array

More Related Videos

Rapid Detection of Helicobacter pylori Virulence and Typing Using Quantum Dot Labeling Technology
05:13

Rapid Detection of Helicobacter pylori Virulence and Typing Using Quantum Dot Labeling Technology

Published on: June 13, 2025

876
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.7K

Related Experiment Videos

Last Updated: May 4, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
Rapid Detection of Helicobacter pylori Virulence and Typing Using Quantum Dot Labeling Technology
05:13

Rapid Detection of Helicobacter pylori Virulence and Typing Using Quantum Dot Labeling Technology

Published on: June 13, 2025

876
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.7K

Area of Science:

  • * Nanotechnology
  • * Biosensors
  • * Quantum Dot Labeling

Background:

  • * Immunoassays are crucial for detecting biomarkers.
  • * Traditional substrates limit sensitivity due to small surface areas.
  • * Quantum dots (QDs) offer bright and photostable fluorescence labeling.

Purpose of the Study:

  • * To develop a high-performance immunoassay platform.
  • * To leverage ZnO nanowire arrays for increased biomolecule immobilization.
  • * To enhance signal detection using QD labeling.

Main Methods:

  • * Fabricated high-density vertical ZnO nanowire arrays.
  • * Utilized photostable quantum dots (QDs) for labeling.
  • * Performed sandwich immunoassays on various substrates, including ZnO nanowires.
  • * Investigated fluorescence resonance energy transfer (FRET) dynamics.
  • * Demonstrated carcinoembryonic antigen (CEA) detection.

Main Results:

  • * ZnO nanowire substrates yielded 3.8x and 8.5x stronger fluorescence signals than ZnO thin film and glass, respectively.
  • * Fluorescence resonance energy transfer (FRET) was suppressed by increasing distance with biotin-streptavidin.
  • * Achieved a low detection limit of 0.001 ng/mL for CEA.
  • * Demonstrated a wide detection range up to 100 ng/mL for CEA.

Conclusions:

  • * The ZnO nanowire array combined with QD labeling creates an efficient immunoassay platform.
  • * This platform significantly enhances fluorescence signal intensity and immunoassay performance.
  • * It shows great potential for sensitive and broad-range biomarker detection.