Lysinibacillus jejuensis sp. nov., isolated from swinery waste

A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain N2-5(T), was isolated from swinery waste collected in Jeju, Republic of Korea. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain N2-5(T) formed a phyletic group within the phylum Firmicutes with less than 97.0% similarities to members of the genus Lysinibacillus, its nearest phylogenetic neighbors. The highest levels of sequence similarity to the isolate were observed against Lysinibacillus xylanilyticus XDB9(T) (96.8%), Lysinibacillus macroides LMG 18474(T) (95.6%), and Lysinibacillus parviboronicapiens BAM-582(T) (95.6%). The organism grew optimally at 30°C and pH 7 and in the presence of 1-3% (w/v) NaCl. Strain N2-5(T) was chemotaxonomically characterized by possessing menaquinone-7 (MK-7) as the major menaquinone, and iso-C15:0 (54.9%), iso-C17:1 ω10c (12.0%), and C16:1 ω7c alcohol (11.8%) as the predominant fatty acids. The genomic DNA G+C content of the novel strain was 43.3 mol% and the cell-wall peptidoglycan was type A4α. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Based on its phenotypic properties and phylogenetic data, strain N2-5(T) (=DSM 28310(T) =KCTC13837(T)) represents a novel species in the genus Lysinibacillus, for which the name Lysinibacillus jejuensis sp. nov. is proposed.