Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tunable electrostatic interactions of lipid-coated quantum dots with biological membranes.

bioRxiv : the preprint server for biology·2026

Same author

AlliGator: Open source fluorescence lifetime imaging analysis in G.

SoftwareX·2026

Same author

High-Performance SiPM Detection Module for Ultra-Fast Time-Resolved Measurements.

Sensors (Basel, Switzerland)·2026

Same author

Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications.

PhotoniX·2025

Same author

Up to 200% of the excitation frequency in compressive FLIM: a constraint-free pile-up correction methodology for advanced biomedical imaging.

Optics express·2025

Same author

Scattering-based super-resolution optical fluctuation imaging.

Optics express·2025

Search research articles

Related Experiment Video

Updated: May 4, 2026

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Published on: February 10, 2017

8-spot smFRET analysis using two 8-pixel SPAD arrays.

Antonino Ingargiola¹, Francesco Panzeri², Niusha Sarkosh¹

¹Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA, USA 90095.

Proceedings of Spie--The International Society for Optical Engineering

|January 4, 2014

Summary

This study enhances single-molecule Förster resonance energy transfer (smFRET) throughput by using multi-spot excitation with multi-pixel SPAD arrays. This advancement enables faster measurements for studying molecular dynamics.

Keywords:

FRET SPAD SPAD arrays high-throughput multi-spot photon-counting single molecule smFRET

More Related Videos

Analysis of Histone Antibody Specificity with Peptide Microarrays

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Related Experiment Videos

Last Updated: May 4, 2026

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Published on: February 10, 2017

Analysis of Histone Antibody Specificity with Peptide Microarrays

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Area of Science:

Biophysics
Biochemistry
Analytical Chemistry

Background:

Single-molecule Förster resonance energy transfer (smFRET) is crucial for biological and biophysical studies.
Current smFRET methods using single confocal spots and single-pixel detectors limit observable dynamics to slow timescales due to long acquisition times.

Purpose of the Study:

To enhance the throughput of smFRET measurements for studying faster molecular dynamics.
To demonstrate a multi-spot excitation approach using multi-pixel SPAD arrays for increased data acquisition.

Main Methods:

Utilized a multi-spot excitation system with two 8-SPAD arrays to collect donor and acceptor photons.
Extended previous work from 4 to 8 independent excitation spots.
Analyzed doubly labeled dsDNA samples with varying donor-acceptor distances.

Main Results:

Achieved an almost tenfold increase in smFRET measurement throughput.
Successfully demonstrated the system's capability across a range of FRET efficiencies.
Validated the approach for studying molecular interactions with faster dynamics.

Conclusions:

The developed multi-spot smFRET system significantly boosts measurement throughput.
This advancement paves the way for investigating rapid single-molecule dynamics.
Future development with larger SPAD arrays will further accelerate smFRET applications.