[The effect of PPARalpha signal channel on atorvastatin inhibiting MMP-9 expression in aging myocytes]
Related Experiment Video
Updated: May 4, 2026
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
Published on: February 20, 2019
Lei Han1, Ping Ye2, Ming-Gao Li3
1Aviation and Nautical Medical Center, Navy General Hospital, Beijing 100048, China. leihan999@sina.com
Objective:
To investigate the correlation between atorvastatin inhibiting the expression level of matrix metalloproteinase 9 (MMP-9) and peroxisome proliferator activated receptor alpha (PPARalpha) signal channel in myocyte of aging rat.
Methods:
Primary cultures of myocyte were got ten from aging rats. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GW6471 group, which treated respectively by cell culture medium, DMSO, atorvastatin, atorvastatin plus GW6471. The expression of MMP-9 mRNA was evaluated by RT-PCR, and content of MMP-9 protein was detected by Western blot.
Results:
(1) There was no difference between control group and DMSO group in level of MMP-9 mRNA and protein expression (P > 0.05); (2) The level of MMP-9 mRNA and MMP-9 protein expression in atorvastatin group were significantly lower than those in control group (P < 0.01); (3) Both level of MMP-9 mRNA and protein expression in atorvastatin plus GW6471 group were significantly higher than those in atorvastatin group (P < 0.05), but were still lower than those in control group (P < 0.05).
Conclusion:
Atorvastatin inhibit MMP-9 expression of aging myocytes by PPARalpha signal channel.
Related Concept Videos
01:18Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Metabolism
01:19The Effect of Aging on Tissues