Related Experiment Video

Updated: Aug 8, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

Selective resistance of bone marrow-derived hemopoietic progenitor cells to gliotoxin

A Müllbacher, D Hume, A W Braithwaite

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1987
    PubMed

    Insights

    The fungal toxin gliotoxin damages DNA in mature lymphocytes but spares resistant bone marrow cells. Gliotoxin-treated bone marrow delayed graft-versus-host disease in mice.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Hematology

    Background:

    • The fungal metabolite gliotoxin inhibits mature lymphocyte stimulation by causing genomic DNA degradation.
    • Bone marrow contains a subpopulation of cells resistant to gliotoxin's DNA-damaging effects.

    Purpose of the Study:

    • To investigate the differential effects of gliotoxin on lymphocytes and bone marrow cells.
    • To evaluate the potential of gliotoxin-resistant bone marrow cells in managing graft-versus-host disease.

    Main Methods:

    • Assessing gliotoxin's impact on lymphocyte mitogen stimulation and DNA integrity.
    • Identifying and characterizing gliotoxin-resistant cell populations within bone marrow.
    • Evaluating the therapeutic potential of gliotoxin-treated bone marrow in allogeneic bone marrow chimeras.

    Main Results:

    • Gliotoxin induced genomic DNA degradation in mature lymphocytes, inhibiting their response to mitogens.
    • A subpopulation of hemopoietic progenitor cells in bone marrow demonstrated resistance to gliotoxin.
    • Treatment with gliotoxin-conditioned lymph node cell-enriched bone marrow significantly delayed graft-versus-host disease onset in allogeneic chimeras.

    Conclusions:

    • Gliotoxin exhibits selective toxicity towards lymphocytes while sparing bone marrow progenitor cells.
    • Gliotoxin-resistant bone marrow cells hold promise for mitigating graft-versus-host disease, a critical complication in bone marrow transplantation.

    More Related Videos

    Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells10:28

    Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells

    Published on: January 10, 2018

    Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts11:15

    Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

    Published on: September 1, 2018

    Related Concept Videos

    Differentiation of Common Myeloid Progenitor Cells01:15

    Differentiation of Common Myeloid Progenitor Cells

    Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...