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A new transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 antibody
1Japanese Red Cross Kinki Block Blood Center, Ibaraki-City, Osaka, Japan.
Background And Objectives:
CD36 antibody (Ab) causes several disorders: neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non-haemolytic transfusion reactions. However, there is no gold-standard test for CD36 Ab.
Materials And Methods:
We developed a transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 Ab and compared it with the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) system in terms of sensitivity and specificity.
Results:
Our new system was characterized by (1) gene-transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD36 MoAbs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system.
Conclusions:
The present results indicate that our new system permits highly sensitive and specific detection of CD36 Ab.
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