[Separation performance of vancomycin-bonded stationary phase].

Area of Science:

• Analytical Chemistry
• Chromatography
• Materials Science

Background:

• Vancomycin, a complex macrocyclic antibiotic, possesses unique functional groups suitable for chromatographic applications.
• Developing versatile stationary phases is crucial for advancing separation science and analytical methodologies.

Purpose of the Study:

• To synthesize and characterize a vancomycin-bonded stationary phase.
• To investigate its chromatographic behavior in reversed-phase, ion-exchange, and hydrophilic interaction modes.
• To demonstrate its application in separating various achiral drugs and stevioside.

Main Methods:

• Preparation of a vancomycin-bonded stationary phase utilizing glutaraldehyde as a spacer.
• Evaluation of chromatographic properties under varying mobile phase compositions (organic solvent content).
• Application of the stationary phase for the separation of eight achiral drugs and stevioside across three distinct chromatographic modes.

Main Results:

• The stationary phase exhibited reversed-phase characteristics at low organic solvent content.
• Chromatographic behavior shifted to hydrophilic interaction mode with increased organic solvent content.
• Ion-exchange properties were observed due to the presence of amino groups in vancomycin.
• Successful separation of target analytes was achieved in all three modes under optimized conditions.

Conclusions:

• The vancomycin-bonded stationary phase demonstrates multi-modal chromatographic capabilities.
• This versatile phase offers a valuable platform for method development in analytical separations.
• The findings guide the design of novel stationary phases modified with complex molecules.