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Luminescent lanthanide cryptates: from the bench to the bedside
Jurriaan M Zwier1, Hervé Bazin, Laurent Lamarque
1Cisbio Bioassays , Parc Marcel Boiteux, BP 84175, Codolet, France.
Inorganic Chemistry
|January 8, 2014
Summary
Luminescent lanthanide cryptates enable sensitive detection of biological interactions. These advanced probes are progressing from in vitro assays to cellular applications and clinical diagnostics using time-resolved fluorescence.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Molecular Medicine
Background:
- Lanthanide cryptates offer unique luminescent properties for bioassays.
- Developing sensitive probes is crucial for understanding biological interactions.
- Homogeneous time-resolved fluorescence (HTRF) technology enhances assay performance.
Purpose of the Study:
- To review the design and application of luminescent lanthanide cryptates for sensing biological interactions.
- To describe the progression from in vitro biochemical assays to cellular and clinical applications.
- To highlight recent advancements in drug discovery and diagnostics using these technologies.
Main Methods:
- Development of luminescent lanthanide macrocyclic compounds.
- Application of antibody-based and suicide-enzyme-based assay technologies.
- Utilizing homogeneous time-resolved fluorescence (HTRF) for sensitive detection.
Main Results:
- Demonstrated utility of lanthanide cryptates in various biological assays.
- Successful transition from in vitro to cellular and potential clinical applications.
- Illustrative examples of drug discovery and diagnostic assays.
Conclusions:
- Luminescent lanthanide cryptates are versatile tools for biological sensing.
- HTRF technology enables sensitive detection for drug discovery and diagnostics.
- Future perspectives include molecular medicine and time-resolved microscopy.
