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Rapid inertial solution exchange (RInSE) enables millisecond-scale chemical treatments for cells and particles. This microfluidic platform enhances molecular biology and cell-based assays through precise control and automation.

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Area of Science:

  • Microfluidics
  • Biotechnology
  • Chemical Engineering

Background:

  • Precise spatiotemporal control of particle and cell interactions is vital for lab and industrial processes.
  • Novel tools for rapid control are needed for new chemical processes and biomedical assays.
  • Rapid Inertial Solution Exchange (RInSE) previously enabled millisecond-scale particle manipulation.

Purpose of the Study:

  • To develop a continuous flow microfluidic platform for transient chemical treatments of cells and particles.
  • To demonstrate the utility of RInSE for molecular biology and automated cell staining.
  • To explore the potential for RInSE in studying molecular event dynamics and improving assay uniformity.

Main Methods:

  • Application of RInSE principles to a continuous flow microfluidic system.
  • Utilizing inertial lift forces for rapid particle and cell transfer between solutions.
  • Employing reactant streams as diffusion barriers to prevent unwanted reactions.

Main Results:

  • Demonstrated millisecond-scale transient chemical treatments of cells and particles.
  • Successfully applied the method to cell permeabilization, fluorescent staining, and molecular delivery.
  • Showcased the reactant stream's effectiveness as a diffusion barrier.

Conclusions:

  • The developed microfluidic platform enables precise, millisecond-scale chemical treatments.
  • RInSE facilitates automation and uniformity in cell-based assays and bead-based reactions.
  • This technology opens new avenues for studying molecular dynamics and developing advanced biomedical assays.