Related Experiment Video
Updated: May 4, 2026
NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
Published on: December 30, 2025
A flower-like nickel oxide nanostructure: synthesis and application for choline sensing
N Sattarahmady1, H Heli2, R Dehdari Vais3
1Pharmaceutical Science Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Flower-like nickel oxide nanostructures were used to create a choline sensor. This novel sensor demonstrates high sensitivity and stability for choline detection, offering a simplified fabrication process.
Area of Science:
- Materials Science
- Electrochemistry
- Nanotechnology
Background:
- Choline detection is crucial in various fields, including clinical diagnostics and environmental monitoring.
- Development of sensitive and stable electrochemical sensors is essential for accurate choline quantification.
- Nickel oxide nanostructures offer promising catalytic properties for electrochemical applications.
Purpose of the Study:
- To synthesize flower-like nickel oxide nanostructures.
- To fabricate a choline sensor using nickel oxide nanostructures as a modifier for a carbon paste electrode.
- To investigate the electrocatalytic oxidation mechanism and kinetics of choline on the modified electrode.
Main Methods:
- Flower-like nickel oxide nanostructures synthesized via desolvation method.
- Fabrication of a carbon paste electrode modified with nickel oxide nanostructures.
- Electrochemical characterization using cyclic voltammetry, steady-state polarization, and chronoamperometry.
Main Results:
- The modified electrode exhibited high electrocatalytic activity and sensitivity towards choline oxidation.
- Key kinetic parameters including catalytic rate constant, charge transfer coefficient, and diffusion coefficient were determined.
- The developed amperometric method achieved a sensitivity of 60.5 mA mol(-1)Lcm(-2) and a limit of detection of 25.4 μmol L(-1).
Conclusions:
- The flower-like nickel oxide nanostructure-modified carbon paste electrode is a highly effective platform for choline sensing.
- The sensor offers advantages of simplicity in fabrication, high performance, and long-term stability.
- This approach provides a promising alternative for sensitive and reliable choline determination without enzyme or reagent immobilization.
