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A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes
Ji Young Eum1, Sang Youn Hwang, Youngjun Ju
1Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea. jbkim3@korea.ac.kr.
Abstract:
A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.

