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Updated: May 4, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Circular permutation of E. coli EPSP synthase: increased inhibitor resistance, improved catalytic activity, and an
Xiongfeng Dai1, Manlu Zhu, Yi-Ping Wang
1State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China. wangyp@pku.edu.cn.
Abstract:
We performed the first circular permutation analysis for E. coli 5-enolpyruvylshikimate-3-phosphate synthase, and identified one circular permutant with notably increased resistance to its specific inhibitor and several others with moderately improved catalytic activity. Valid circular permutation sites can be used as effective split sites of protein fragment complementation.
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