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Glottic regeneration with a tissue-engineering technique, using acellular extracellular matrix scaffold in a canine
Morimasa Kitamura1, Shigeru Hirano2, Shin-Ichi Kanemaru3,4
1Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Japan. m_kitamura@ent.kuhp.kyoto-u.ac.jp.
Journal of Tissue Engineering and Regenerative Medicine
|January 10, 2014
Summary
Porcine urinary bladder matrix (UBM) scaffolds show promise for hemilarynx regeneration in dogs. While UBM is biocompatible and aids tissue repair, vocal fold scarring remains a challenge.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Otolaryngology
Background:
- Acellular extracellular matrix (ECM) scaffolds, like porcine urinary bladder matrix (UBM), are utilized for tissue regeneration.
- Previous studies demonstrated UBM's efficacy in repairing various tissues and organs.
Purpose of the Study:
- To evaluate the regenerative potential of UBM scaffolds in a canine hemilarynx defect model.
- To assess functional and histological outcomes following UBM implantation for laryngeal reconstruction.
Main Methods:
- Canine model underwent left partial hemilaryngectomy with UBM scaffold reconstruction.
- Functional assessment included phonation threshold pressure (PTP), normalized mucosal wave amplitude (NMWA), and normalized glottal gap (NGG) at 6 months.
- Histological analysis examined scaffold integration and tissue remodeling.
Main Results:
- Good re-epithelialization observed within 1 month, with minimal complications.
- Functional tests showed near-normal PTP in two cases and normal NMWA in three cases.
- Histology revealed scaffold biocompatibility and integration, with new cartilage formation, but significant vocal fold scarring.
Conclusions:
- UBM scaffolds are biocompatible and biodegradable, supporting hemilarynx tissue regeneration.
- UBM shows potential for restoring vocal fold function, though mucosal scarring requires further investigation.
- Further research is needed to address vocal fold mucosal scarring for improved functional outcomes.
