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Updated: May 4, 2026
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
A fluorogenic probe with aggregation-induced emission characteristics for carboxylesterase assay through formation of
Xiaojing Wang1, Huan Liu, Jingwen Li
1College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123 (China), Tel: (+86) 0512-65882917.
Abstract:
Herein, we report a novel fluorescent "light-up" probe useful for carboxylesterase assay that is based on a tetraphenylethylene derivative containing carboxylic ester groups. The specific cleavage of the carboxylic ester bonds by carboxylesterase results in the generation of a relatively hydrophobic moiety that self-assembles into supramolecular microfibers, thus giving rise to "turn-on" fluorescent signals. A high sensitivity towards carboxylesterase was achieved with a detection limit as low as 29 pM, which is much lower than the corresponding assays based on other fluorescent approaches.
