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Updated: May 4, 2026
A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
Published on: October 13, 2019
New fluoranthene FLUN-550 as a fluorescent probe for selective staining and quantification of intracellular lipid
Atul Goel1, Ashutosh Sharma, Manoj Kathuria
1Fluorescent Lab, Division of Medicinal and Process Chemistry, ‡Electron Microscopy Unit, §Division of Pharmaceutics, and ∥Division of Toxicology, CSIR-Central Drug Research Institute (CDRI) , Lucknow 226031, India.
Abstract:
A new class of live cell permeant, nontoxic fluoranthene-based fluorescent probe (FLUN-550) having a high Stokes shift in aqueous medium has been discovered. It showed selective staining of lipid droplets (LDs, dynamic cytoplasmic organelles) at a low concentration without background noise in in vitro live cell imaging of 3T3-L1 preadipocytes, J774 macrophages, MCF7 breast cancer cells, and single-celled, parasitic protozoa Leishmania donovani promastigotes and in vivo nonparasitic soil nematode C. elegans.
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