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Updated: May 4, 2026

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
Published on: February 10, 2016
Nanoscopy of filamentous actin in cortical dendrites of a living mouse
Katrin I Willig1, Heinz Steffens2, Carola Gregor2
1Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen, Germany.
Abstract:
We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 μm in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43-70 nm resolution reveal dynamical changes in spine morphology.
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