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Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Identification of rye chromosomes: the Giemsa banding pattern and the translocation tester set
1Dept. of Genetics, University of Agriculture, Wageningen, The Netherlands.
Abstract:
The Giemsa banding pattern is given for eleven reciprocal translocations of rye, Secale cereale L., together involving all chromosomes at least once, and one telocentric substitution. It is possible to correlate the identification system based on the Giemsa pattern with that based on the translocation tester set. The location of the translocation break points could be determined very exactly for a number of translocations, somewhat less exactly for others. The variations in the banding pattern, resulting from genetic, environmental and technical variation, make definite identification with the nomenclature system of the different rye additions to wheat difficult. An attempt is made, but some caution is necessary.

