Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk of gastric or peritoneal recurrence, and long-term outcomes, following pancreatic cancer resection with preoperative endosonographically guided fine needle aspiration.

Endoscopy·2013
Same author

Analysis of related factors on the deformity correction of balloon kyphoplasty.

AJNR. American journal of neuroradiology·2013
Same author

Family-based association of an ANK3 haplotype with bipolar disorder in Latino populations.

Translational psychiatry·2013
Same author

Observation of associated near-side and away-side long-range correlations in sqrt[s(NN)]=5.02 TeV proton-lead collisions with the ATLAS detector.

Physical review letters·2013
Same author

Search for dark matter annihilations in the sun with the 79-string IceCube detector.

Physical review letters·2013
Same author

Distribution of androgen and oestrogen receptors-α in the seminal vesicle-related spinal neurones in male rats.

Journal of neuroendocrinology·2013

Related Experiment Video

Updated: May 4, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

707

Recent Advances in Fiber Lasers for Nonlinear Microscopy.

C Xu1, F W Wise1

  • 1School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853.

Nature Photonics
|January 14, 2014
PubMed
Summary

Recent advances in fiber lasers are enhancing nonlinear microscopy for biological imaging. These developments promise more robust and affordable instruments for advanced bioimaging applications.

Area of Science:

  • Biomedical Engineering
  • Optics
  • Microscopy

Background:

  • Nonlinear microscopy has revolutionized biological imaging over the last 20 years.
  • Solid-state femtosecond lasers have been the primary technology driving these advancements.

Purpose of the Study:

  • To discuss recent progress in fiber lasers for nonlinear bioimaging.
  • To identify challenges and opportunities for the integration of fiber lasers into bioimaging instruments.

Main Methods:

  • Review of recent advances in fiber laser technology.
  • Analysis of the suitability of fiber lasers for nonlinear microscopy applications.

Main Results:

  • Fiber lasers present attractive features for biological and biomedical imaging.

More Related Videos

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

7.0K
Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
12:54

Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Published on: July 17, 2016

10.6K

Related Experiment Videos

Last Updated: May 4, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

707
Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

7.0K
Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
12:54

Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Published on: July 17, 2016

10.6K
  • Recent advancements are enabling high-performance fiber laser sources.
  • These sources have the potential for robust, inexpensive, and integrated bioimaging instruments.

    • Conclusions:

    • Fiber lasers are a promising technology for the future of nonlinear bioimaging.
    • Further development is needed to overcome challenges and fully realize opportunities.