Related Experiment Video
Updated: May 4, 2026
Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
Published on: November 1, 2024
[High-quality total RNA extraction from medicinal plant Aquilaria sinensis]
Hong-Qing Wu1, Lei Wang2, Wei Guo2
1State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Open Laboratory of Applied Microbiology/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application/Guangdong Institute of Microbiology, Guangzhou 510070, China. whq0401@163.com
Objective:
It is quite difficult to isolate total RNA from Aquilaria sinensis due to the complexity of its secondary metabolites. In order to obtain the high-quality RNA, we investigated sampling method and RNA extraction from both normal and agilawood tissues of the plant.
Methods:
Five methods such as modified CTAB-LiCl method, modified CTAB-LiCl method with no water-bath, SDS-acid phenol method, modified guanidinium thiocyanate-CTAB method and EASYspin RN09 RNA isolation kit were used to extract RNA from leaves, branches, white wood samples, agilawood samples of A. sinensis, respectively.
Results:
Total RNA was stable for 5 hours at room temperature after extracted from A. sinensis branches. EASYspin RN09 RNA isolation kit was the best extraction method for the leaf while modified guanidinium thiocyanate-CTAB method was the best for the other samples.
Conclusion:
A great quantity of RNAs can be isolated from samples of white wood and agilawood wood, respectively. Their ratios of 28S : 18S are in the range of 1.2 - 1.4 and RINs are between 6.8 - 7.5. These RNAs can be applied in transcriptome sequencing.
