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[Study on UPLC specific chromatogram of Lily and its specific peaks compositions analysis by QTOF-MS]
Hui Nie1, Hui Yan2, Da-Wei Qian2
1School of Pharmacy, Jiangsu University, Zhenjiang 212013, China. 2009niehui@sina.com
Objective:
To establish the UPLC specific chromatogram of Lily and analyze the specific peaks compositions by ESI-QTOF-MS.
Methods:
The samples were conducted by ACQUITY UPLC BEH C18 Column (2.1 mm x 100 mm, 1.7 microm) and eluted with acetonitrile and 0.1% formic acid at the flow rate of 0.4 mL/min. The detection wavelength was set at 320 nm and column temperature was 35 degrees C. Negative ion mode was chosen for qualitative analysis. The capillary voltage was set at 3.0 kV. The nebulization gas was set to 600 L/h at 350 degrees C, and the source temperature was 120 degrees C.
Results:
The specific chromatogram of Lily was obtained. There were 19 common peaks. Twelve phenylpropenoid glycerides compositions were identified. Among them, 6 compositions were identified by comparison with the reference substances and others were identified by MS and MS2 data.
Conclusion:
UPLC specific chromatogram can be used for the quality evaluation of Lily, giving support to quality control comprehensively.
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