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Partial purification and properties of pectin lyase from Penicillium expansum
D O Silva1, M M Attwood, D W Tempest
1Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, P.O. Box 594, S10 2UH, Sheffield, UK.
Abstract:
A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.10, from a culture filtrate of Penicillium expansum was partially purified 33-fold with 7.3% yield. The enzyme was monomeric with a molecular mass of 36.5 kDa. The enzyme did not contain pectate lyase activity and degraded citrus and apple pectin best at pH 7.0 and 40 to 45°C. The K m for citrus pectin was 9 mg ml(-1).
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