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Lysis of mammalian and Sf9 cells
Jennifer M Kavran1, Daniel J Leahy1
1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods in Enzymology
|January 16, 2014
Abstract:
Use a French press to disrupt mammalian or Sf9 cells and generate a clarified lysate for subsequent use in protein purification.
