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Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

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High laccase producing mutants ofPleurotus florida.

R P Dhaliwal¹, H S Garcha, P K Khanna

¹Department of Microbiology, Punjab Agricultural University, 141 004, Ludhiana, India.

World Journal of Microbiology & Biotechnology

|January 16, 2014

Summary

Pleurotus florida mushrooms naturally produce high levels of the enzyme laccase. Researchers developed hyperlaccase mutants for enhanced enzyme production, showing similar growth patterns to the original mushroom strain.

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Area of Science:

Enzymology
Mycology
Biotechnology

Background:

Laccase is an enzyme with diverse industrial applications.
Pleurotus florida is a known laccase producer.
Optimizing laccase production is crucial for biotechnological use.

Purpose of the Study:

To quantify laccase production in Pleurotus florida.
To develop hyperlaccase mutants of Pleurotus florida.
To assess the characteristics of these hyperlaccase mutants.

Main Methods:

Cultivating Pleurotus florida in malt extract broth under stationary conditions.
Inducing mutagenesis in mycelial protoplasts using N-methyl-N'-nitro-N-nitrosoguanidine.
Selecting and evaluating hyperlaccase mutants based on growth and enzyme production.

Main Results:

Pleurotus florida yielded high laccase amounts (4.60 U/ml) after 12 days.
Laccase production in the parent strain was semi-constitutive.
Three hyperlaccase mutants were successfully generated, exhibiting comparable growth and enzyme production to the wild type.

Conclusions:

Pleurotus florida is an efficient source of laccase.
Mutagenesis is a viable strategy for enhancing laccase production.
Hyperlaccase mutants retain desirable growth characteristics.