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Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
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Purification and viability determinations of plant protoplasts
1School of Botany, University of New South Wales, 2033, Kensington, N.S.W., Australia.
Planta
|January 17, 2014
Abstract:
A method is described for purifying plant protoplasts from cellular and subcellular debris. The procedure utilizes a density buffer containing 9.6% sodium metrizoate and 5.6% Ficoll. The use of fluorescein diacetate for assessing the viability of plant protoplasts is also reported.
