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X-ray-amorphous calcium phosphate (ACP) synthesis in a simple biomineralization medium.

Journal of materials chemistry. B·2020
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Brushite (CaHPO<sub>4</sub>·2H<sub>2</sub>O) to octacalcium phosphate (Ca<sub>8</sub>(HPO<sub>4</sub>)<sub>2</sub>(PO<sub>4</sub>)<sub>4</sub>·5H<sub>2</sub>O) transformation in DMEM solutions at 36.5°C.

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Grade-1 titanium soaked in a DMEM solution at 37°C.

A Cuneyt Tas1

  • 1Department of Materials Science and Engineering, University of Illinois, Urbana, IL 61801, USA.

Materials Science & Engineering. C, Materials for Biological Applications
|January 18, 2014
PubMed
Summary

Researchers used a novel Dulbecco

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Culture Technology

Background:

  • Dulbecco's modified Eagle medium (DMEM) is crucial for in vitro cell culture and biomaterial biocompatibility assessment.
  • Previous studies on DMEM-based biomimetic coatings often used formulations with phenol red and sodium pyruvate, yielding different results.
  • Alkali-treated titanium (Ti) is a promising biomaterial, but its surface interactions require further investigation.

Purpose of the Study:

  • To investigate the biomimetic coating of alkali-treated grade-1 titanium substrates using a specific Hepes-buffered, phenol red- and sodium pyruvate-free DMEM.
  • To compare the coating results with previous studies using different DMEM formulations.
  • To explore the use of a simulated inorganic solution for biomimetic coating.

Main Methods:

Keywords:
AmorphousCalcium phosphateDMEMTitanium

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  • Immersion of alkali-treated grade-1 Ti substrates in Hepes-buffered, phenol red- and sodium pyruvate-free DMEM at 37°C.
  • Analysis of deposited coatings using techniques such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS).
  • Utilizing a simulated inorganic solution with similar ion concentrations to DMEM for coating experiments.

Main Results:

  • Deposition of X-ray-amorphous calcium phosphate (ACP) on grade-1 Ti substrates within one to two weeks of immersion in the specialized DMEM.
  • Comparison with previous studies showing cryptocrystalline apatitic calcium phosphate (Ap-CaP) deposition when using different DMEM formulations.
  • Successful deposition of ACP on grade-1 Ti substrates within 24 hours using the simulated inorganic solution.

Conclusions:

  • The formulation of DMEM significantly influences the type of calcium phosphate deposit formed on alkali-treated titanium.
  • Hepes-buffered, phenol red- and sodium pyruvate-free DMEM promotes the formation of amorphous calcium phosphate (ACP).
  • A simulated inorganic solution can effectively induce ACP deposition on titanium, offering a simplified approach.