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Updated: May 3, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The complete mitochondrial genome of Microhyla pulchra (Amphidia, Anura, Microhylidae)
Xiaoyou Wu1, Yongmin Li1,2, Huabin Zhang1,3
1a Anhui Province Key Laboratory for Conservation and Exploitation of Biological Resource , College of Life Sciences, Anhui Normal University , Wuhu , China .
Abstract:
The complete mitochondrial genome of Microhyl pulchra was determined in this work. This mitogenome was 16,744 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region (CR). The following four distinctive features were observed: a protein-coding gene (ND1) began with GTG as start codon; eight protein-coding genes (ND1, COII, ATP6, COIII, ND3, ND4, ND5 and Cytb) ended with incomplete stop codon T; four tRNA genes positions (tRNA-Leu (CUN)/tRNA-Thr/Trna-Pro/tRNA-Phe) located between CR and 12S rRNA genes, which was a novel mtDNA gene rearrangement in amphibians; there was no significant repeat regions in the CR.
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