Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

143
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
143
The Proteasome Structure01:17

The Proteasome Structure

2.2K
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
2.2K
The Proteasome02:18

The Proteasome

7.8K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
7.8K
The Proteasome01:13

The Proteasome

1.6K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.6K
The Proteasome02:18

The Proteasome

3.9K
3.9K
Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond <i>difficile</i>: three novel toxin B-producing <i>Clostridioides</i> species from human patients with diarrhea.

Emerging microbes & infections·2026
Same author

Community context reshapes microbial proteomes and reduces functional overlap.

Nature microbiology·2026
Same author

The outer membrane vesicle-associated peptidyl-arginine deiminase of Porphyromonas gingivalis is required for macrophage evasion and modulates blood-brain barrier passage in vitro.

Microbiological research·2026
Same author

Scanning-Based Dynamic Mask Projection for Ultrafast Laser Ablation of Thin Films.

Nanomaterials (Basel, Switzerland)·2026
Same author

Fructan utilization by members of marine Gammaproteobacteria involves SusC/D-like proteins.

The ISME journal·2026
Same author

Impact of SliP4 deletion on the high-light acclimation in <i>Synechocystis</i> sp. PCC 6803.

microLife·2026

Related Experiment Video

Updated: May 3, 2026

A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

9.0K

The Staphylococcus aureus proteome.

Andreas Otto1, Jan Maarten van Dijl2, Michael Hecker1

  • 1Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Friedrich-Ludwig-Jahn-Str. 15, D-17489 Greifswald, Germany.

International Journal of Medical Microbiology : IJMM
|January 21, 2014
PubMed
Summary
This summary is machine-generated.

Staphylococcus aureus poses a major healthcare threat due to antibiotic resistance and virulence. Proteomics research is advancing our understanding of its stress responses and resistance mechanisms.

Keywords:
2D gel electrophoresisBiofilmsEntire proteomeMS-based proteomicsMembrane vesiclesPhysiological proteomicsStress/starvation responsesSurfacomeVirulence factors

More Related Videos

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.3K
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
12:27

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Published on: January 3, 2020

8.4K

Related Experiment Videos

Last Updated: May 3, 2026

A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

9.0K
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.3K
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
12:27

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Published on: January 3, 2020

8.4K

Area of Science:

  • Microbiology
  • Proteomics
  • Bacterial Pathogenesis

Background:

  • Staphylococcus aureus is a significant healthcare threat due to antibiotic resistance and virulence factors.
  • Understanding its resistance mechanisms and stress responses is crucial for developing new therapies.

Purpose of the Study:

  • To review recent advances in Staphylococcus aureus proteomics.
  • To highlight the importance of location-specific and integrated proteome studies.
  • To discuss emerging topics in staphylococcal proteomics.

Main Methods:

  • Gel-based and gel-free proteomics analyses.
  • Location-specific proteomics (cytosol, membrane, cell surface, extracellular milieu).
  • Integrated global proteome studies.

Main Results:

  • Recent advances in proteomics provide insights into S. aureus.
  • Location-specific studies are vital for a comprehensive understanding.
  • Emerging areas like in vivo proteomics and biofilm studies are promising.

Conclusions:

  • Proteomics is essential for unraveling S. aureus resistance and virulence.
  • Integrated and location-specific approaches are key to future discoveries.
  • Further research in areas like systems biology modeling is warranted.