Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein C functional assay using snake venom activator.

I V Nathan1, H Ping, M M Pradhan

  • 1Department of Pediatrics, National University of Singapore.

Thrombosis Research
|July 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A bio-process inspired synthesis of vaterite (CaCO<sub>3</sub>), directed by a rationally designed multifunctional protein, ChiCaSifi.

Journal of materials chemistry. B·2020
Same author

Prevalence of afebrile malaria and development of risk-scores for gradation of villages: A study from a hot-spot in Odisha.

PloS one·2019
Same author

Comprehensive case management of malaria: Operational research informing policy.

Journal of vector borne diseases·2019
Same author

Hormonal Changes in Intracranial Hemorrhage.

JNMA; journal of the Nepal Medical Association·2017
Same author

Response of malaria vectors to conventional insecticides in the southern districts of Odisha State, India.

The Indian journal of medical research·2014
Same author

Search for the rare decays K(L)→π0π0μ+μ- and K(L)→π0π0X0→π0π0μ+μ-.

Physical review letters·2011
Same journal

Apoptotic versus procoagulant platelets: similar "necrotic" phenotype and procoagulant activity in vitro, but distinct adhesive protein composition.

Thrombosis research·2026
Same journal

Heatstroke-induced coagulopathy: A scoping review of therapeutic strategies and outcome reporting.

Thrombosis research·2026
Same journal

Mapping thrombus habitat: Non-contrast MRI radiomics and pixel-tile histomics approach to track venous thrombosis evolution in mice.

Thrombosis research·2026
Same journal

A study protocol for a randomised controlled trial evaluating the safety and efficiency of the YEARS algorithm versus computed tomography pulmonary angiography only for suspected acute pulmonary embolism in patients with cancer: the Hydra Study.

Thrombosis research·2026
Same journal

Associating the phenotypic expression of platelets with disease type through image-based single-cell profiling.

Thrombosis research·2026
Same journal

The mechanisms of contractile dysfunction following chronic limited platelet activation in (pro)thrombotic conditions.

Thrombosis research·2026
See all related articles

A novel assay using Protac-C snake venom measures functional Protein C levels in whole plasma. This simple method correlates well with ELISA and is suitable for high-throughput screening.

Area of Science:

  • Biochemistry
  • Hematology
  • Clinical Chemistry

Background:

  • Protein C is a crucial vitamin K-dependent anticoagulant protein.
  • Accurate measurement of functional Protein C is essential for assessing thrombotic risk and monitoring anticoagulant therapy.
  • Existing functional assays can be complex and time-consuming.

Purpose of the Study:

  • To develop and validate a simple, whole-plasma functional assay for Protein C using Protac-C.
  • To evaluate the assay's correlation with established methods like ELISA.
  • To assess Protein C levels in healthy volunteers and warfarinized patients.

Main Methods:

  • A novel functional assay utilizing Protac-C, a snake venom activator, was employed.
  • The assay was performed on whole plasma, eliminating the need for absorption-elution steps.

Related Experiment Videos

  • The method was adapted for a microtitre plate system to facilitate high-throughput sample testing.

    • Main Results:

    • The Protac-C functional assay demonstrated good correlation (r = 0.8) with antigenic Protein C levels measured by ELISA.
    • In 58 healthy volunteers, mean functional Protein C levels were 82%, and mean antigenic levels were 95.5%.
    • Warfarinized patient samples exhibited lower mean Protein C levels compared to healthy controls.

    Conclusions:

    • The Protac-C assay provides a simple, efficient, and reliable method for determining functional Protein C levels in whole plasma.
    • This assay is suitable for large-scale sample testing and may aid in the clinical assessment of Protein C deficiency and anticoagulant monitoring.
    • The findings highlight the utility of snake venom-derived activators in developing practical diagnostic assays for coagulation factors.