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Area of Science:

  • Food safety and water quality analysis
  • Virology and molecular diagnostics
  • Public health and risk assessment

Background:

  • Molecular methods like RT-qPCR are standard for detecting enteric viruses (e.g., norovirus, hepatitis A) in food and water.
  • Current detection requires specific sampling and sample preparation, which may differ from bacterial plans.
  • A standardized reference method (ISO/TS 15216) is crucial for evaluating and validating new virus detection techniques.

Purpose of the Study:

  • To highlight the significance of the ISO/TS 15216 standard for virus detection method development.
  • To address the challenge of correlating molecular detection results with viral infectivity.
  • To emphasize the need for pretreatment methods that can distinguish infectious from non-infectious viruses.

Main Methods:

  • Review and discussion of the implications of the ISO/TS 15216 standard for enteric virus detection.
  • Analysis of current limitations in molecular virus detection, particularly regarding sample preparation and infectivity assessment.
  • Exploration of the need for advanced techniques to determine viral infectivity post-detection.

Main Results:

  • The ISO/TS 15216 standard provides a framework for evaluating virus detection methods, improving comparability and validation.
  • Standardized sample preparation is essential for reliable molecular detection of enteric viruses.
  • A significant gap exists in differentiating between infectious and non-infectious viral particles detected by molecular methods.

Conclusions:

  • The development of a reference method is a key step towards reliable enteric virus detection in food and water.
  • Future research should focus on developing methods to assess viral infectivity to enhance risk assessment accuracy.
  • Improved molecular detection strategies, including sample preparation and infectivity assays, are vital for public health protection.