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Updated: May 3, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Continuous throughput and long-term observation of single-molecule FRET without immobilization
Swati Tyagi1, Virginia VanDelinder2, Niccolò Banterle3
11] Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. [2].
Abstract:
We present an automated microfluidic platform that performs multisecond observation of single molecules with millisecond time resolution while bypassing the need for immobilization procedures. With this system, we confine biomolecules to a thin excitation field by reversibly collapsing microchannels to nanochannels. We demonstrate the power of our method by studying a variety of complex nucleic acid and protein systems, including DNA Holliday junctions, nucleosomes and human transglutaminase 2.

