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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
Changes in plastid envelope polypeptides during chloroplast development
1Department of Biological Sciences, University of Lancaster, LA1 4YQ, Lancaster, UK.
Abstract:
A quantitative estimation of sodium dodecyl sulphate-extractable plastid envelope polypeptides during greening of Avena sativa L. laminae is described, combined with protein distribution and plastid number studies over the same period.A primary light-dependent and cytoplasm-dependent increase in both total plastid protein and envelope associated protein during the first 30 minutes of greening was observed, followed by a period during which release of envelope-associated protein either into the plastid or into the cytoplasm, or both, takes place. After 8 hours greening an increase in plastid envelope protein reflects a requirement for an increased plastid surface area during chloroplast maturation.A comparison of the behaviour of envelope membranes during illuminated in vitro incubation of isolated etio-plasts with plastids isolated from tissue given similar illumination pre-treatments, indicates that the early increase in envelope-associated protein is real and requires the participation of the cytoplasm.
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