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CELLULAR READJUSTMENT OF BARLEY SEEDLINGS TO SIMULATED ACID RAIN.

Jennifer Wolfenden1, A R Wellburn1

  • 1Department of Biological Sciences, University of Lancaster, Lancaster LA1 5YQ, UK.

The New Phytologist
|April 20, 2021
PubMed
Summary
This summary is machine-generated.

Short-term acid exposure in barley seedlings did not alter buffer capacity but increased plastidic sulfate. Vacuolar pH decreased, suggesting energy costs for plant homeostatic adjustments to acidity.

Keywords:
31P-nuclear magnetic resonanceHordeum vulgarechloroplastsion chromatographyvacuolar pH

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Area of Science:

  • Plant Physiology
  • Environmental Science
  • Biochemistry

Background:

  • Acidic precipitation can impact plant health.
  • Understanding plant responses to short-term acid stress is crucial.

Purpose of the Study:

  • To investigate the physiological and biochemical responses of barley seedlings to short-term acidic conditions.
  • To determine if acid exposure affects buffer capacity, ion levels, and vacuolar pH.

Main Methods:

  • Hydroponic growth of barley seedlings with varying acidic treatments (pH 3-4) and acidic mist spraying.
  • Analysis of buffer capacity, plastidic sulfate and other anions using high performance ion chromatography.
  • Measurement of vacuolar pH in barley shoot tissue using 31P-NMR spectroscopy.

Main Results:

  • No significant changes in buffer capacity were observed in shoots or roots under acidic conditions.
  • Acidic treatments led to a significant increase in plastidic sulfate levels.
  • Vacuolar pH decreased in barley shoots sprayed with acidic solutions, indicating potential involvement of ATP-dependent H+-pumps.

Conclusions:

  • Short-term acid exposure induces biochemical changes in barley seedlings, including increased plastidic sulfate and decreased vacuolar pH.
  • These homeostatic adjustments may incur an energetic cost, potentially impacting plant growth.
  • The findings suggest a mechanism for growth reduction observed under acidic precipitation.