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Updated: May 3, 2026

Author Spotlight: Studying Cardiac Cell-Matrix Interactions In Vitro
Published on: March 22, 2024
Role of extracellular matrix signaling cues in modulating cell fate commitment for cardiovascular tissue engineering
Karina H Nakayama1, Luqia Hou, Ngan F Huang
1Department of Cardiothoracic Surgery, Stanford University, 300 Pasteur Dr, Stanford, CA, 94305, USA; Cardiovascular Institute, Stanford University, 265 Campus Drive, G1120, MC-5454, Stanford, CA, 94305, USA; Veterans Affairs Palo Alto Health Care System, 3801 Miranda Avenue, Mail Code 153, Palo Alto, CA, 94304 60031l, 650-493-5000, USA.
Abstract:
It is generally agreed that engineered cardiovascular tissues require cellular interactions with the local milieu. Within the microenvironment, the extracellular matrix (ECM) is an important support structure that provides dynamic signaling cues in part through its chemical, physical, and mechanical properties. In response to ECM factors, cells activate biochemical and mechanotransduction pathways that modulate their survival, growth, migration, differentiation, and function. This Review describes the role of ECM chemical composition, spatial patterning, and mechanical stimulation in the specification of cardiovascular lineages, with a focus on stem cell differentiation, direct transdifferentiation, and endothelial-to-mesenchymal transition. The translational application of ECMs is discussed in the context of cardiovascular tissue engineering and regenerative medicine.
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