Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Promoter efficiency depends upon intragenic sequences.

A M Koltunow1, K Gregg, G E Rogers

  • 1Department of Biochemistry, University of Adelaide, SA, Australia.

Nucleic Acids Research
|October 12, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

UL54 foscarnet mutation in an hematopoietic stem cell transplant recipient with cytomegalovirus disease.

Transplant infectious disease : an official journal of the Transplantation Society·2014
Same author

A program to facilitate retention of employment among persons with multiple sclerosis.

Work (Reading, Mass.)·2014
Same author

A multicenter evaluation of pandemic influenza A/H1N1 in hematopoietic stem cell transplant recipients.

Transplant infectious disease : an official journal of the Transplantation Society·2013
Same author

Consumption of a high-salt diet by ewes during pregnancy alters nephrogenesis in 5-month-old offspring.

Animal : an international journal of animal bioscience·2012
Same author

The influence of MHC and immunoglobulins a and e on host resistance to gastrointestinal nematodes in sheep.

Journal of parasitology research·2011
Same author

Risk assessment of porcine reproductive and respiratory syndrome virus (PRRSV) transmission via somatic cell nuclear transfer (SCNT) embryo production using oocytes from commercial abattoirs.

Animal reproduction science·2011
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

The Herpes Simplex Virus Thymidine Kinase (HSV-TK) promoter

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Xenopus Oocyte Research

Background:

  • Gene transcription efficiency is crucial for cellular function.
  • Promoter activity can be influenced by adjacent DNA sequences.
  • Understanding these interactions is key to gene expression studies.

Purpose of the Study:

  • To investigate the influence of gene sequences on the HSV-TK promoter's efficiency.
  • To determine if the TK gene itself affects promoter activity.
  • To characterize the nature of this interaction.

Main Methods:

  • Utilizing Xenopus oocytes as a model system.
  • Cloning and attaching different gene sequences (e.g., avian keratin, chicken histone H2B) to the HSV-TK promoter.
  • Measuring transcriptional efficiency using reporter gene assays.

Related Experiment Videos

  • Analyzing the impact of promoter-gene sequence proximity and orientation.

    • Main Results:

    • The HSV-TK promoter's efficiency was significantly higher (30-fold) when linked to its own gene compared to other genes.
    • Attaching the keratin gene promoter to the TK gene increased keratin promoter efficiency by 20-fold.
    • The TK gene demonstrated a cis-acting stimulatory effect on adjacent promoters.
    • This effect was independent of orientation but sensitive to the distance between sequences.

    Conclusions:

    • Gene sequences significantly modulate promoter efficiency, specifically impacting the HSV-TK promoter.
    • The TK gene possesses intrinsic properties that enhance the activity of nearby promoters.
    • This cis-regulatory effect highlights the complex interplay between gene sequences and transcriptional machinery.