Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Biomarkers to Biosensors: Transforming Comorbidity Management in Dialysis Care.

Sensors (Basel, Switzerland)·2026
Same author

3D ECM-inflammation model on a microfluidic chip for neutrophil transmigration from whole blood investigations.

Lab on a chip·2026
Same author

A portable, low-cost, point-of-care DNA amplification kit with impedance-based detection for decentralized antimicrobial resistance diagnostics.

Lab on a chip·2026
Same author

Virus Genome Sequences in the Blood of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients.

medRxiv : the preprint server for health sciences·2025
Same author

Enabling multiple cellular enumeration applications of a bioparticle sensing platform using machine learning.

Analytical methods : advancing methods and applications·2025
Same author

Wireless Power-Up and Readout of Label-Free Nanosensors for In-Vivo Monitoring of Protein Concentrations in Live Animals.

Langmuir : the ACS journal of surfaces and colloids·2025
Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

9.8K

Digital microfluidic assay for protein detection.

Janine Mok1, Michael N Mindrinos, Ronald W Davis

  • 1Stanford Genome Technology Center, Department of Biochemistry, Stanford University, Palo Alto, CA 94304.

Proceedings of the National Academy of Sciences of the United States of America
|January 23, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed a low-cost digital microfluidic platform for sensitive protein biomarker detection. This portable device offers a cost-effective alternative to ELISA for point-of-care diagnostics, enabling early disease detection.

Keywords:
bead-based assaybiosensordecoupled architecture

More Related Videos

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

12.2K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.2K

Related Experiment Videos

Last Updated: May 3, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

9.8K
Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

12.2K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.2K

Area of Science:

  • Biotechnology
  • Proteomics
  • Microfluidics

Background:

  • Global proteomic studies have identified numerous protein biomarkers for disease detection.
  • Current clinical application of these biomarkers is limited by the lack of sensitive, portable, and low-cost detection methods.
  • Enzyme-Linked Immunosorbent Assay (ELISA) is the gold standard but relies on bulky, non-portable equipment.

Purpose of the Study:

  • To develop a novel digital microfluidic platform for sensitive, low-cost protein biomarker detection.
  • To create a portable device suitable for point-of-care diagnostics.
  • To demonstrate the platform's ability to quantify both protein abundance and activity.

Main Methods:

  • Development of a digital microfluidic platform utilizing simple electronics for portability.
  • Adaptation of the platform for assaying diverse proteomic biomarkers.
  • Quantification of Interleukin-6 (IL-6) abundance and Abelson tyrosine kinase (Abelson-TK) activity.

Main Results:

  • The digital microfluidic platform achieved high sensitivity, detecting Interleukin-6 (IL-6) at concentrations as low as 50 pM.
  • Sensitivity for IL-6 was an order of magnitude higher than comparable laboratory-designed ELISAs.
  • Abelson tyrosine kinase (Abelson-TK) activity was detectable at 100 pM concentrations.
  • The platform requires minimal sample volume (<5 μL).

Conclusions:

  • The developed digital microfluidic platform offers a sensitive, low-cost, and portable solution for protein biomarker detection.
  • This technology has the potential to overcome limitations of current methods, enabling point-of-care diagnostics and early disease detection.
  • The platform's adaptability allows for the detection of various proteomic biomarkers, including both abundance and activity.