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Updated: Aug 13, 2026

Staining Proteins in Gels
Published on: July 8, 2008
Fluorescent staining of proteins transferred to nitrocellulose allowing for subsequent probing with antisera
1Department of Cellular, Viral, and Molecular Biology, University of Utah, Salt Lake City 84132.
Abstract:
A sensitive staining method for protein blots on nitrocellulose is described. It is based on the coupling of a fluorochrome, dichlorotriazynylaminofluorescein, to protein which yields products colorless in visible light but colored when protein blots are illuminated with long-range ultraviolet light. The coupling of a fluorochrome does not affect the antigenic properties of proteins and the stained blots can be subsequently probed with antisera. Thus, the method allows for the unambiguous identification of antigenic proteins transferred to nitrocellulose from sodium dodecyl sulfate-polyacrylamide gels.
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