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Updated: May 3, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
[Effects of nitrate on anoxic/anaerobic oxidation of methane in the aged refuse]
Yan-Yan Liu1, Yan Long2, Hua Yin3
1College of Science and Engineering, Jinan University, Guangzhou 510632, China. 124917785@qq.com
Abstract:
This work investigated the effects of nitrate on anoxic/anaerobic methane oxidation in the aged refuse landfilled 10-12 years by full-factor experiments. The results showed that under anoxic/anaerobic condition, nitrate could promote the removal of methane in the aged refuse. The contents of CH4 and NO3(-) -N had significant influences on the removal of CH4 and the generation of N2, and there were significant interactions as well (P < 0.05). The increasing initial CH4 content evidently enhanced the removal of CH4 and the production of N2. Furthermore, the addition of NO3(-) -N obviously improved the de-nitrification process. The results suggested that nitrate reduction could be coupled to anoxic/anaerobic oxidation of methane in the aged refuse. In the experimental condition, the effort of coupling was most effective when the concentrations of CH4 and nitrate were 30% and 110 mg x kg(-1), respectively.
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