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The isolation and characterization of murine monoclonal antibodies directed to polymorphic epitopes on HLA antigens
J P Johnson1, I Contag, R Wank
1Institute for Immunology, Munich, FRG.
Twenty-two murine monoclonal antibodies directed to distinct polymorphic epitopes on HLA class I or class II antigens have been isolated and characterized using a simple protocol for fusion and hybridoma selection. Thirteen MAbs directed to class I antigens are reported here for the first time. The majority of these MAbs reacted with multiple specificities, often revealing a surprising sharing of epitopes. MAbs directed against single classically defined alloantigenic specificities were also obtained.
Twenty-two murine monoclonal antibodies directed to distinct polymorphic epitopes on HLA class I or class II antigens have been isolated and characterized using a simple protocol for fusion and hybridoma selection. Thirteen MAbs directed to class I antigens are reported here for the first time. The majority of these MAbs reacted with multiple specificities, often revealing a surprising sharing of epitopes. MAbs directed against single classically defined alloantigenic specificities were also obtained.