Related Experiment Video
Updated: May 3, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Published on: March 13, 2015
Blood alcohol stability in postmortem blood samples
Davorka Sutlovic1, Maja Versic-Bratincevic, Marija Definis-Gojanovic
1From the *Department of Pathology and Forensic Medicine, University Hospital Centre Split; and †Department Forensic Medicine, University of Split School of Medicine, Split, Croatia.
Abstract:
The effect of long-term storage on alcohol stability in postmortem blood samples was investigated. Seventy-nine blood samples were used in this study. For the first time, blood alcohol concentrations were measured within 1 to 4 days after being taken at the Laboratory of Forensic Toxicology. After storage (ranging from 191 to 468 days), each sample was reanalyzed for blood alcohol concentration, and results were compared with the original analysis. Results showed good agreement between the experimental measurements. About 90% of the results lie within 95% limits, and 10% were outside. Despite these positive correlations, the results of some blood samples showed a high variation in ethanol concentration. Deviation in alcohol concentration between replicates of up to 10% (observed in 39% samples) is not acceptable as precise forensic evidence.

