The isoenzyme distribution of etiolated pea seedling lipoxygenase

Acetone powders from etiolated dwarf pea seedlings (Pisum sativum.) contain lipoxygenase activity that can be separated by carboxymethylcellulose chromatography into 5 active fractions. The total lipoxygenase activity and the distribution of activity among the fractions changes with seedling age. Both stem and leaf tissue contain lipoxygenase activity. Two active fractions are specific to leaf tissue, one to stem tissue, while the other two fractions are common to both. These isoenzyme distributions are compared with that in pea seeds.