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Updated: May 3, 2026

Isolation and Whole-Cell Patch-Clamp Recording of Hippocampal Microglia from Adult Mice
Published on: September 27, 2024
Voltage-gated proton channel HV1 in microglia
1Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA lwu@dls.rutgers.edu.
The voltage-gated proton channel HV1 in microglia plays a key role in brain inflammation and damage. Inhibiting microglial HV1 may offer a new therapeutic strategy for stroke and other neurological diseases.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Microglia are crucial immune cells in the brain, involved in both health and disease.
- Microglia express various ion channels, including the voltage-gated proton channel HV1, essential for their function.
- The role of microglial HV1 in brain pathophysiology remains largely unexplored.
Purpose of the Study:
- To review the discovery, properties, regulation, and pathological roles of the microglial HV1 proton channel.
- To highlight the significance of HV1 in microglial function and brain injury.
Main Methods:
- Literature review of studies on microglial ion channels, focusing on HV1.
- Analysis of cellular and in vivo data regarding HV1 expression and function in the brain.
- Examination of mouse models of ischemic stroke to assess HV1's contribution to brain damage.
Main Results:
- HV1 is selectively expressed in brain microglia, not neurons.
- Microglial HV1 regulates intracellular pH and supports NADPH oxidase-dependent reactive oxygen species generation.
- In a stroke model, microglial HV1 exacerbates neuronal death and ischemic brain injury.
Conclusions:
- Microglial HV1 is a critical regulator of microglial activity and contributes to ischemic brain damage.
- Targeting microglial HV1 presents a potential therapeutic avenue for neurological disorders involving neuroinflammation.
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