Using pig manure to promote fermentation of sugarcane molasses alcohol wastewater and its effects on microbial
Peihong Shen1, Fei Han2, Shuquan Su3
1College of Life Science and Technology of Guangxi University, Nanning 530005, Guangxi, China; State Key Laboratory for Conservation and Utilization of Agricultural Bioresources in the Subtropics, Nanning 530005, Guangxi, China.
Abstract:
Molasses alcohol wastewater (MAW) is difficult to be bio-treated and converted into biogas. In this study, MAW mixed with pig manure (PM) in different ratios was co-digested. Biogas production, chemical oxygen demand (COD) removal and the structure of microbial communities were monitored in the process. Our results showed that under the optimal COD ratio of PM:MAW (1.0:1.5), CODremoval and biogas yield were the highest. And in fermentation tanks with different PM to MAW ratios, the structure and composition of bacterial communities varied in the early and late stage. Furthermore, the type of main bacterial operational taxonomic units (OTUs) have no differences, yet the relative abundance of OTUs varied. The current research showed that there was a good potential to the use of PM as a co-digested material to anaerobic treatment of MAW and provided references for further improving bio-treatment of MAW.
Insights
Co-digesting molasses alcohol wastewater (MAW) with pig manure (PM) enhances biogas production and chemical oxygen demand (COD) removal. Optimal results were achieved at a PM:MAW ratio of 1.0:1.5, improving anaerobic treatment efficiency.
Area of Science:
- Environmental Microbiology
- Biotechnology
- Waste Management
Background:
- Molasses alcohol wastewater (MAW) presents significant challenges for conventional biological treatment and biogas conversion.
- Anaerobic digestion is a promising technology for treating organic waste, but MAW's recalcitrance limits its efficiency.
- Co-digestion with readily biodegradable materials can potentially enhance the anaerobic treatment of difficult wastewaters.
Purpose of the Study:
- To investigate the feasibility and effectiveness of co-digesting molasses alcohol wastewater (MAW) with pig manure (PM).
- To optimize the ratio of PM to MAW for enhanced biogas production and chemical oxygen demand (COD) removal.
- To analyze the impact of co-digestion on the microbial community structure during anaerobic treatment.
Main Methods:
- Co-digestion experiments were conducted using MAW and PM at various ratios.
- Biogas production and chemical oxygen demand (COD) removal were monitored throughout the digestion process.
- Bacterial community structures were analyzed using 16S rRNA gene sequencing to identify operational taxonomic units (OTUs).
Main Results:
- The optimal ratio for co-digestion was determined to be a COD ratio of 1.0:1.5 for PM:MAW, yielding the highest COD removal and biogas yield.
- Significant variations in the structure and composition of bacterial communities were observed between the early and late stages of fermentation across different PM:MAW ratios.
- While the types of dominant bacterial operational taxonomic units (OTUs) remained consistent, their relative abundance varied significantly with the co-digestion ratio and stage.
Conclusions:
- Pig manure (PM) is a viable and effective co-substrate for the anaerobic treatment of molasses alcohol wastewater (MAW).
- Co-digestion significantly improves the efficiency of biogas production and COD removal from MAW.
- Understanding the shifts in microbial community dynamics is crucial for optimizing anaerobic digestion processes for challenging industrial wastewaters.
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