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Effects of storage time and temperature on coagulation tests and factors in fresh plasma
Limin Feng1, Ying Zhao1, Hongcan Zhao2
1Department of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Abstract:
Coagulation tests and factors measurements have been widely applied in clinical practice. Pre-analytical conditions are very important in laboratory assessment.Here,we aim to determine the effects of storage time and temperature on activated partial thromboplastin time (APTT), fibrinogen (Fbg), prothrombin time (PT), the international normalized ratio (INR), thrombin time (TT), factor VIII activity (FVIII:C), and factor IX activity (FIX:C) in fresh plasma. Seventy-two blood samples were tested after storage for 0 (baseline), 2, 4, 6, 8, 12, and 24 h at 25°C (room temperature) and 4°C (refrigeration) in two centers. The mean percentage change of greater than 10% and the numbers of samples with greater than 10% percentage changes more than 25% were used to determine clinically relevant difference. We demonstrated that samples for Fbg, PT/INR, and TT could be safely stored for ≤24 h; FVIII:C for ≤2 h; FIX:C for ≤4 h both at 4°C and 25°C; and APTT for ≤12 h at 4°C and ≤8 h at 25°C.
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