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Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
Published on: November 28, 2016
The value and distribution of high-density lipoprotein subclass in patients with acute coronary syndrome
Li Tian1, Chuanwei Li2, Yinghui Liu1
1Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
Insights
In patients with acute coronary syndrome (ACS), small high-density lipoprotein (HDL) subclasses increase while large ones decrease. This HDL subclass remodeling is linked to metabolic factors and may aid ACS risk stratification.
Area of Science:
- Cardiovascular Medicine
- Lipid Metabolism
- Biomarker Discovery
Background:
- High-density lipoprotein (HDL) possesses anti-atherosclerosis properties.
- The clinical benefit of elevated HDL in acute coronary syndrome (ACS) remains debated.
- This study investigates HDL subclass distribution in ACS patients.
Purpose of the Study:
- To examine the HDL subclass distribution profile in patients with acute coronary syndrome (ACS).
- To identify factors influencing HDL subclass metabolism in ACS.
- To explore the potential of HDL subclass phenotypes as biomarkers for ACS risk stratification.
Main Methods:
- Plasma HDL subclasses were quantified in 158 ACS patients using two-dimensional gel electrophoresis and immunoblotting.
- ACS diagnosis was confirmed by clinical symptoms, ECG, cardiac enzymes, and coronary angiography.
- Correlations between HDL subclasses and clinical/biochemical parameters were analyzed.
Main Results:
- ACS patients showed higher levels of small preβ1-HDL, HDL3b, and HDL3a, and lower levels of large HDL2a and HDL2b compared to controls.
- HDL2b decreased and preβ1-HDL increased with elevated LDL-C, FPG, BMI, and BP in ACS patients.
- Apolipoprotein A-I positively correlated with all HDL subclasses; FPG and total cholesterol inversely associated with large HDL subclasses.
Conclusions:
- A distinct remodeling of HDL subclass distribution occurs in ACS patients.
- Lipoprotein levels, fasting plasma glucose (FPG), blood pressure (BP), and body mass index (BMI) significantly impact HDL subclass metabolism in ACS.
- HDL subclass phenotypes may serve as novel biomarkers for ACS risk stratification.
Background:
High-density lipoprotein (HDL) enhances cholesterol efflux from the arterial wall and exhibits potent anti-inflammatory and anti-atherosclerosis (AS) properties. Whether raised HDL levels will clinically benefit patients with acute coronary syndrome (ACS) and the value at which these effects will be apparent, however, is debatable. This study examined the HDL subclass distribution profile in patients with ACS.
Methods:
Plasma HDL subclasses were measured in 158 patients with established ACS and quantified by two-dimensional gel electrophoresis and immunoblotting. ACS diagnosis was based on symptoms of cardiac ischemia, electrocardiogram (ECG) abnormalities, speciality cardiac enzyme change along with presence of coronary heart disease (CHD) on coronary angiography.
Results:
The small-sized preβ1-HDL, HDL3b, and HDL3a levels were significantly higher, and the large-sized HDL2a and HDL2b levels were significantly lower in patients with ACS than in those with stable angina pectoris (SAP) and in normal control subjects. Meanwhile, with an elevation in the low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), body mass index (BMI), and blood pressure (BP), and the reduction in the high density lipoprotein cholesterol (HDL-C) levels, the HDL2b contents significantly decreased and the preβ1-HDL contents significantly increased in patients with ACS. The correlation analysis revealed that the apolipoprotein (apo)A-I levels were positively and significantly with all HDL subclasses contents; plasma total cholesterol (TC) and fasting plasma glucose (FPG) levels were inversely associated with HDL2a, and HDL2b. Moreover, the FPG levels were positively related to HDL3c, HDL3b, and HDL3a in ACS patients.
Conclusion:
The HDL subclass distribution profile remodeling was noted in the patients with ACS. Plasma lipoprotein and FPG levels, BP, and BMI play an important role in the HDL subclass metabolism disorder for patients with ACS. The HDL subclass distribution phenotype might be useful as a novel biomarker to assist in the risk stratification of patients with ACS.
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