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[Correlation between the changes of oxidation reduction potential values and postmortem interval of heart blood in
Tian-tong Yang1, Yong-guang Yu2, Jing Bai2
1China University of Political Science and Law, Key Laboratory of Evidence Science, Ministry of Education, Beijing 100040, China. yangtiantong@126.com
Objective:
To investigate correlation between the changes of oxidation reduction potential (ORP) values of heart blood in rabbits after death and postmortem interval (PMI) at different temperatures.
Methods:
Forty-eight rabbits were randomly divided into 6 groups and sacrificed by air embolism. Blood samples were taken from the right ventricle of each rabbit and stored at different temperatures of 10, 15, 20, 25, 30 and 35 degrees C, respectively. Every 4 hours from 0 h to 132 h postmortem, the ORP values of the blood samples were measured at different intervals by PB-21 electrochemical analyzer. The curvilinear regression equation was established by SPSS 17.0 software. The surface equation and 3D surface diagram were established by MATLAB 7.10.0 software.
Results:
The ORP values at different temperatures of heart blood in rabbits were highly correlated with the PMI. The ORP values rised obviously when the temperature was high and rised slowly when the temperature was low. The surface equation and 3D surface diagram were obtained.
Conclusion:
The surface equation and 3D surface diagram of ORP values and PMI may be used for PMI estimation at different temperatures.
