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Updated: May 3, 2026

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
Published on: June 20, 2019
Decomposition of size-exclusion chromatography elution curves of complex branched polymers
Miloš Netopilík1, Miroslav Janata1
1Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Square 2, 162 06 Prague 6, Czech Republic.
Abstract:
A new method for the decomposition of non-baseline-resolved multimodal elution curves of SEC with the concentration, light scattering and viscosity detection is presented. The method makes possible the characterization of the polymer-sample components, represented by the peaks forming multimodal elution curves, individually and reduces also the error in the calculation of molecular-weight averages. The procedure is demonstrated on narrow molecular-weight distribution polystyrene standards and their mixture as well as on a grafted polymer sample.
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