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Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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Related Experiment Video

Updated: May 3, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
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Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

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JAK-STAT3 and somatic cell reprogramming.

Yong Tang1, Xiuchun Cindy Tian1

  • 1Center for Regenerative Biology; Department of Animal Science; University of Connecticut; Storrs, CT USA.

JAK-STAT
|January 29, 2014
PubMed
Summary
This summary is machine-generated.

Leukemia inhibitory factor (LIF) signaling, particularly the JAK-STAT3 pathway, is crucial for maintaining pluripotency in stem cells. Understanding this pathway is key for advancing induced pluripotent stem cell (iPSC) technology and regenerative medicine.

Keywords:
JAKLIFSTAT3embryonic stem cellsepigeneticsiPSCreprogramming

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In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors
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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

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Related Experiment Videos

Last Updated: May 3, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
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In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors
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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

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Area of Science:

  • Stem cell biology
  • Molecular signaling pathways
  • Regenerative medicine

Background:

  • Induced pluripotent stem cell (iPSC) technology is vital for generating diverse stem cells for research and regenerative medicine.
  • The precise mechanisms of somatic cell reprogramming remain incompletely understood.
  • Leukemia inhibitory factor (LIF) signaling is critical for maintaining pluripotency in mouse embryonic stem cells (ESCs) and iPSCs.

Purpose of the Study:

  • To review the role of the LIF signaling pathway in ESC pluripotency and somatic cell reprogramming.
  • To elucidate the function of the Janus kinase-signal transducer and activator of transcription 3 (JAK-STAT3) pathway in this process.
  • To highlight the importance of understanding these pathways for developing patient-specific stem cell therapies.

Main Methods:

  • Literature review of historical and recent research on LIF signaling and pluripotency.
  • Focus on the JAK-STAT3 pathway's involvement in maintaining ground-state pluripotency.
  • Analysis of LIF's regulatory role in ESC pluripotency maintenance and somatic cell reprogramming.

Main Results:

  • LIF signaling, via the JAK-STAT3 pathway, is essential for maintaining the ground-state pluripotency of mouse ESCs and iPSCs.
  • Detailed characterization of the JAK-STAT3 pathway is fundamental to generating naïve pluripotent human iPSCs.
  • This understanding is a prerequisite for advancing patient-specific stem cell therapy.

Conclusions:

  • The LIF-JAK-STAT3 signaling axis is a central regulator of pluripotency in stem cells.
  • Further elucidation of this pathway is critical for the successful application of iPSC technology in regenerative medicine.
  • Targeting LIF signaling may unlock new avenues for generating and utilizing human pluripotent stem cells.