Calcium extrusion regulatory molecules: differential expression during pregnancy in the porcine uterus

Calcium ions in the uterine endometrium are essential for the establishment and maintenance of pregnancy, but the cellular and molecular mechanisms of calcium ion regulation in the endometrium are not fully understood. Our previous study in pigs found that calcium regulatory molecules, transient receptor potential, vanilloid type 6 and calbindin-D9K, are expressed in the uterine endometrium during the estrous cycle and pregnancy. However, we did not determine the expression of calcium extrusion regulatory molecules, plasma membrane calcium ATPases (ATP2Bs), sodium/calcium exchangers (SLC8As), or potassium-dependent sodium/calcium exchangers (SLC24As), in the uterine endometrium and conceptuses. Thus, in this study we determine whether ATP2Bs, SCL8As, and SLC24As are expressed in the uterine endometrium during the estrous cycle and pregnancy and in conceptuses during early pregnancy. Real-time RT-PCR analysis showed that ATP2Bs, SLC8As, and SLC24As were expressed in the uterine endometrium in a pregnancy status- and stage-specific manner. Conceptuses during early pregnancy also expressed these molecules. In situ hybridization analysis showed that ATP2B1, SLC8A1, and SLC24A4 were localized mainly to luminal and glandular epithelium and stromal cells in the endometrium during pregnancy. These results indicate that calcium extrusion regulatory molecules are expressed in the uterine endometrium during the estrous cycle and pregnancy and in conceptuses during early pregnancy, indicating that calcium extrusion regulatory molecules may play important roles in the establishment and maintenance of pregnancy by regulating calcium ion concentration in the uterine endometrium in pigs.