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Related Experiment Videos

Rapid axonal transport in focally demyelinated sciatic nerve.

R Armstrong1, A D Toews, P Morell

  • 1Biological Sciences Research Center, University of North Carolina, Chapel Hill 27514.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|December 1, 1987
PubMed
Summary

Acute demyelination in rat sciatic nerves did not alter the transport or deposition of newly synthesized macromolecules. Axonal transport rates and protein composition remained unchanged, suggesting intact axonal function despite myelin loss.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Demyelination, a hallmark of neurological disorders, affects nerve function.
  • Understanding macromolecule transport in demyelinated axons is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the impact of acute focal demyelination on axonal transport of proteins and glycoproteins.
  • To determine if demyelination alters the synthesis, transport rates, or deposition of newly synthesized macromolecules.

Main Methods:

  • Induced focal demyelination in rat sciatic nerves using anti-galactocerebroside serum.
  • Assessed nerve conduction block to confirm functional lesion.
  • Quantified radioactivity of 35S-methionine and 3H-fucose labeled proteins and glycoproteins in nerve segments over time.

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  • Analyzed transported macromolecules using polyacrylamide gel electrophoresis and light microscopic autoradiography.

    • Main Results:

    • Demyelination affected 50-70% of nerve fibers, with intact axonal structures.
    • Axonal transport rates (approx. 260 mm/d) and the composition of transported proteins/glycoproteins were not altered in demyelinated nerves.
    • Autoradiography showed similar localization of labeled macromolecules in both demyelinated and control axons, primarily within axons initially and along axolemmal regions later.

    Conclusions:

    • Acute focal primary demyelination does not significantly alter the axonal transport or deposition of newly synthesized macromolecules.
    • Axonal transport machinery appears resilient to acute myelin damage.
    • Findings suggest that therapeutic strategies targeting macromolecule transport may not be directly impacted by acute demyelination.