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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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Related Experiment Video

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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
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Development of an antigen microarray for high throughput monoclonal antibody selection.

Nicole Staudt1, Nicole Müller-Sienerth1, Gavin J Wright1

  • 1Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom.

Biochemical and Biophysical Research Communications
|January 30, 2014
PubMed
Summary

Researchers developed a new method for selecting monoclonal antibodies, enabling the screening of more antibody candidates against multiple antigens simultaneously. This streamlined approach improves efficiency in identifying functional antibodies for various applications.

Keywords:
High throughputHybridomaMonoclonal antibodiesProtein microarray

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Area of Science:

  • Biotechnology
  • Immunology
  • Molecular Biology

Background:

  • Monoclonal antibodies (mAbs) are vital research tools and therapeutic agents.
  • Selecting validated mAbs for specific applications is challenging and resource-intensive.
  • Existing methods face limitations in screening capacity and efficiency.

Purpose of the Study:

  • To develop an improved method for early screening and selection of monoclonal antibodies.
  • To overcome limitations in antigen number and supernatant volume for pooled immunisation.
  • To streamline the process of identifying functional antibodies against multiple antigens.

Main Methods:

  • Development of an antigen microarray for reduced supernatant screening.
  • Integration of hybridoma technology with refined screening and cloning techniques.
  • Utilisation of small-scale transfection for rapid identification of functional antibody-encoding plasmids.

Main Results:

  • The antigen microarray significantly reduces the supernatant volume needed for screening.
  • The approach allows for a substantial increase in the number of antigens for parallel mAb selection.
  • A convenient small-scale transfection method efficiently identifies functional cloned antibodies.

Conclusions:

  • A hybrid approach combining hybridoma technology with refined screening and cloning methods is effective.
  • This method enables the selection of functional monoclonal antibodies against multiple antigens from a single host.
  • The developed strategy enhances efficiency and expands the scope of monoclonal antibody discovery.